{"id":18742,"date":"2026-03-30T09:16:35","date_gmt":"2026-03-30T09:16:35","guid":{"rendered":"https:\/\/www.diginerve.com\/blogs\/?p=18742"},"modified":"2026-03-30T09:16:35","modified_gmt":"2026-03-30T09:16:35","slug":"biochemistry-important-questions-neet-pg-2026","status":"publish","type":"post","link":"https:\/\/www.diginerve.com\/blogs\/biochemistry-important-questions-neet-pg-2026\/","title":{"rendered":"Biochemistry Important Questions for NEET PG 2026"},"content":{"rendered":"<p><span style=\"font-weight: 400;\">Practising Biochemistry important questions for <a href=\"https:\/\/www.diginerve.com\/blogs\/neet-pg-important-dates-eligibility-criteria-application-process-admit-card\/\"><strong>NEET PG 2026<\/strong><\/a> is essential because recent exams focus heavily on clinical integration, enzyme defects, metabolic pathways, and genetics.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">These questions are designed based on previous year questions (PYQs), recent trends, and high-yield topics.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q1. A child presents with severe fasting hypoglycemia, hepatomegaly, and lactic acidosis. Which enzyme is deficient?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Glycogen synthase<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Glucose-6-phosphatase<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Hexokinase<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Pyruvate kinase<\/span><\/p>\n<p><b>Answer: B. Glucose-6-phosphatase<\/b><\/p>\n<p><span style=\"font-weight: 400;\">This is Von Gierke disease (Glycogen storage disease type I). Deficiency of glucose-6-phosphatase leads to:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Severe fasting hypoglycemia<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Lactic acidosis<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Hepatomegaly<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><b>Q2. A patient has haemolytic anaemia due to decreased ATP production in RBCs. Which enzyme is deficient?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Hexokinase<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Pyruvate kinase<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Lactate dehydrogenase<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Glucose-6-phosphate dehydrogenase<\/span><\/p>\n<p><b>Answer: B. Pyruvate kinase<\/b><\/p>\n<p><span style=\"font-weight: 400;\">RBCs depend on glycolysis for ATP. Pyruvate kinase deficiency \u2192 \u2193 ATP \u2192 membrane instability \u2192 hemolysis.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q3. A child presents with developmental delay and a cherry-red spot on the retina. Which is the most likely diagnosis?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Gaucher disease<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Tay-Sachs disease<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Niemann-Pick disease<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Fabry disease<\/span><\/p>\n<p><b>Answer: B. Tay-Sachs disease<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Tay-Sachs is due to <\/span><b>hexosaminidase A deficiency,<\/b><span style=\"font-weight: 400;\"> leading to GM2 ganglioside accumulation.<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">Key features:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Neurodegeneration<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Cherry-red spot<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">No hepatosplenomegaly<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><b>Q4. A patient with obesity has increased levels of which lipoprotein?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. HDL<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. LDL<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. VLDL<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Chylomicrons<\/span><\/p>\n<p><b>Answer: C. VLDL<\/b><\/p>\n<p><span style=\"font-weight: 400;\">VLDL carries endogenous triglycerides and is elevated in obesity and metabolic syndrome.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q5. A newborn presents with vomiting, lethargy, and a musty odour to the urine. Diagnosis?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Alkaptonuria<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Phenylketonuria<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Maple syrup urine disease<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Homocystinuria<\/span><\/p>\n<p><b>Answer: B. Phenylketonuria<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Phenylketonuria (PKU) is due to <\/span><b>phenylalanine hydroxylase deficiency<\/b><span style=\"font-weight: 400;\">:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Musty odor<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Intellectual disability<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Elevated phenylalanine<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><b>Q6. Maple syrup urine disease is due to a deficiency of:<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Branched-chain amino acid dehydrogenase<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Phenylalanine hydroxylase<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Tyrosinase<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Homogentisate oxidase<\/span><\/p>\n<p><b>Answer: A<\/b><\/p>\n<p><span style=\"font-weight: 400;\">MSUD involves defective metabolism of:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Leucine<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Isoleucine<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Valine<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">Leading to <\/span><b>sweet-smelling urine and neurological symptoms<\/b><span style=\"font-weight: 400;\">.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q7. Which enzyme shows zero-order kinetics at high substrate concentration?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Allosteric enzyme<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Michaelis-Menten enzyme<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Competitive enzyme<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Non-competitive enzyme<\/span><\/p>\n<p><b>Answer: B. Michaelis-Menten enzyme<\/b><\/p>\n<p><span style=\"font-weight: 400;\">At high substrate concentration, enzymes become saturated \u2192 reaction rate becomes constant \u2192 zero-order kinetics.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q8. A competitive inhibitor affects which parameter?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Decreases Vmax<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Increases Vmax<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Increases Km<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Decreases Km<\/span><\/p>\n<p><b>Answer: C. Increases Km<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Competitive inhibition:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Km increases (lower affinity)<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Vmax remains unchanged<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><b>Q9. Which enzyme is responsible for removing RNA primers during DNA replication?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. DNA polymerase I<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. DNA polymerase II<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. DNA polymerase III<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Helicase<\/span><\/p>\n<p><b>Answer: A. DNA polymerase I<\/b><\/p>\n<p><span style=\"font-weight: 400;\">DNA polymerase I has <\/span><b>exonuclease activity<\/b><span style=\"font-weight: 400;\"> that removes RNA primers and fills gaps.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q10. In which direction does DNA synthesis occur?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. 3&#8242; \u2192 5&#8242;<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. 5&#8242; \u2192 3&#8242;<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Both directions<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Random<\/span><\/p>\n<p><b>Answer: B. 5&#8242; \u2192 3&#8242;<\/b><\/p>\n<p><span style=\"font-weight: 400;\">DNA polymerase adds nucleotides only in the <\/span><b>5&#8242; to 3&#8242; direction<\/b><span style=\"font-weight: 400;\">.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q11. A patient presents with bleeding gums and poor wound healing. Deficiency of:<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Vitamin A<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Vitamin C<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Vitamin D<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Vitamin K<\/span><\/p>\n<p><b>Answer: B. Vitamin C<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Vitamin C is required for <\/span><b>collagen synthesis<\/b><span style=\"font-weight: 400;\">. Deficiency \u2192 scurvy:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Bleeding gums<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Poor healing<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><b>Q12. Night blindness is due to a deficiency of:<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Vitamin A<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Vitamin D<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Vitamin E<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Vitamin K<\/span><\/p>\n<p><b>Answer: A. Vitamin A<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Vitamin A is essential for <\/span><b>rhodopsin formation<\/b><span style=\"font-weight: 400;\"> in the retina.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q13. A patient with liver disease shows increased ammonia levels. Which pathway is affected?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Glycolysis<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Urea cycle<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. TCA cycle<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Pentose phosphate pathway<\/span><\/p>\n<p><b>Answer: B. Urea cycle<\/b><\/p>\n<p><span style=\"font-weight: 400;\">The liver converts ammonia to urea. Liver failure \u2192 hyperammonemia \u2192 encephalopathy.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q14. A patient presents with gout. Which metabolite is elevated?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Urea<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Creatinine<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Uric acid<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Ammonia<\/span><\/p>\n<p><b>Answer: C. Uric acid<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Gout results from <\/span><b>uric acid accumulation<\/b><span style=\"font-weight: 400;\"> due to a purine metabolism disorder.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q15. Rate-limiting enzyme of glycolysis:<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Hexokinase<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Phosphofructokinase-1<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Pyruvate kinase<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Glucose-6-phosphatase<\/span><\/p>\n<p><b>Answer: B<\/b><\/p>\n<p><span style=\"font-weight: 400;\">PFK-1 is the <\/span><b>key regulatory enzyme<\/b><span style=\"font-weight: 400;\"> of glycolysis.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q16. NADH is produced in:<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Glycolysis<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. TCA cycle<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Beta-oxidation<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. All of the above<\/span><\/p>\n<p><b>Answer: D<\/b><\/p>\n<p><span style=\"font-weight: 400;\">NADH is generated in multiple pathways, including glycolysis, the TCA cycle, and fatty acid oxidation.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q17. Which vitamin acts as a coenzyme in transamination reactions?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Vitamin B1<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Vitamin B6<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Vitamin B12<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Vitamin C<\/span><\/p>\n<p><b>Answer: B. Vitamin B6<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Pyridoxine (B6) is required for <\/span><b>transamination reactions<\/b><span style=\"font-weight: 400;\">.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q18. In oxidative phosphorylation, ATP is generated by:<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Substrate-level phosphorylation<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Electron transport chain<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Glycolysis<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Fermentation<\/span><\/p>\n<p><b>Answer: B<\/b><\/p>\n<p><span style=\"font-weight: 400;\">ATP is produced via <\/span><b>oxidative phosphorylation in mitochondria<\/b><span style=\"font-weight: 400;\">.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q19. A patient with G6PD deficiency develops hemolysis after drug intake. Cause?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. ATP deficiency<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. Increased oxidative stress<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Decreased haemoglobin<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Increased glucose<\/span><\/p>\n<p><b>Answer: B<\/b><span style=\"font-weight: 400;\">G6PD produces NADPH \u2192 protects RBCs from oxidative damage. Deficiency \u2192 hemolysis.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>Q20. Which pathway produces NADPH?<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A. Glycolysis<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">B. TCA cycle<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">C. Pentose phosphate pathway<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><span style=\"font-weight: 400;\">D. Urea cycle<\/span><\/p>\n<p><b>Answer: C<\/b><\/p>\n<p><span style=\"font-weight: 400;\">PPP generates NADPH for:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Fatty acid synthesis<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Antioxidant defense<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">These Biochemistry important questions for NEET PG 2026 reflect:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Clinical case-based learning<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">High-yield enzyme and pathway concepts<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">PYQ-focused patterns<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">To score well:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Focus on metabolic pathways and their regulation<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Practice clinical MCQs regularly<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Revise enzymes, vitamins, and genetics thoroughly<\/span><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Practising Biochemistry important questions for NEET PG 2026 is essential [&hellip;]<\/p>\n","protected":false},"author":16,"featured_media":17273,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[412],"tags":[937,938,939],"class_list":["post-18742","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-biochemistry","tag-biochemistry-important-questions","tag-biochemistry-mcqs","tag-neet-pg-biochemistry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - 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